A Secret Weapon For flow cytometers
A Secret Weapon For flow cytometers
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Information-enriched characterization of cell populations. Morphological information from pictures can insert to your richness of flow cytometry info. For instance, the determine reveals an in any other case conventional apoptosis assay applying Annexin V and PI, introducing cell imaging to characterize cells in Every single inhabitants to reveal morphologically unique options. These insights couldn't have already been acquired from multiplex staining on your own.
As samples are obtained on the Attune CytPix Flow Cytometer, the substantial-pace brightfield digicam captures and suppliers pictures of detected activities at a fee as much as 6,000 pictures for each 2nd, based on the flow amount and graphic size. For greater versatility, Attune Cytometric Software package permits you to regulate the graphic capture frequency as essential and choose a certain gate for picture capture, if essential.
Combine acoustic focusing technological innovation with large-parameter Assessment for adaptable spectral unmixing and conventional workflows.
Learn new insights into your experimental information by visualizing populations applying blended fluorescence and morphology info. Our computer software interface wizards help downsampling, concatenation (merged documents) and dimensionality resources which have been designed in to the software package for an intuitive, streamlined data Evaluation practical experience.
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Additionally, it really is illuminating to view the quantity of doublets slip as a result of classic more info singlet gates, and great to verify the position of sudden double-positive events in my Evaluation."
Pair your Attune flow cytometer using an autosampler for improved workflow performance, and add robotic integration for maximum throughput.
Our experts will design and style panels for you as often times you may need—gratis, no solution order required.
Early indications of this contamination had at first been dismissed as debris. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also enables you to use morphological capabilities to discover interesting subpopulations that could not be apparent from flow cytometry information alone.
People can view function visuals in true-time to confirm sample quality, enhance protocols, and file the morphology of every mobile populace discovered for Evaluation. Imaging seize also Positive aspects from acoustic focusing, which really helps to posture the cells in order that a pointy, centered graphic is received.
Audio waves enable exact cell alignment, resulting in Excellent info good quality and ~10X faster sample flow costs.
Application Notice: Automatic image Assessment cuts down consumer-to-user variability in flow cytometry gating approaches
Constructed-in dimensionality reduction resources elevated resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify envisioned inhabitants resolution in lysed entire human blood. Consumer can view situations from Each and every cluster for visual confirmation of cell phenotypes.
Adaptable payment strategy from Agilent Monetary Remedies help you receive the significant instrumentation you need for rapid task, or to increase your lab.
Gating on these extended parameters permits you to promptly and accurately discover populations of curiosity and confirm gating method with little or no handbook evaluation. The Attune software package offers access to state-of-the-art device Understanding analysis resources in an expertly intended person interface, which makes it exceptionally convenient to use.
As the cell line had previously been analyzed about the Attune CytPix Flow Cytometer, the researchers went back again to the photographs and ended up in the position to doc the microbial infection no less than five times before.
The Attune CytPix Flow Cytometer allows people to visualise samples throughout acquisition, validate the precision of gating methods, and support outcomes with trusted documentation for publication applications.
Below, we clearly show that by gating tactics making use of image-primarily based quantitative parameters can distinguish interacting cells from coincident events to extra properly evaluate interacting cells.